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monarch hmw dna extraction kit  (New England Biolabs)


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    New England Biolabs monarch hmw dna extraction kit
    Summary of ONT sequencing experiment results. ( A ) Comparison of the N50 value and the total output of each sequencing run of the four analyzed samples (in different colors). Diverse sequencing protocols using whole-genome sequencing (WGS) or adaptive sampling (AS2/AS3) and <t>DNA</t> isolation methods (Monarch <t>HMW</t> or phenol) are represented by different shapes, whereas empty symbols indicate the use of fresh cells as starting material. ( B ) Enrichment ratio between the read depth inside and outside the targeted regions (depth in/out) of each sample for diverse types of sequencing (black dots), showing a six- to sevenfold average enrichment in AS2 or AS3 compared with WGS (colored bars). ( C ) Average proportion of genotypes obtained for the analyzed inversion regions normalized to the number of AS2 or AS3 targets (percentage, colored bars). Values for each sample (black dots) show considerable variation due mainly to sequencing output differences.
    Monarch Hmw Dna Extraction Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+extraction+kit/Monarch+HMW+DNA+Extraction+Kit+for+Cells+%26+Blood/pmc13629690-134-15-24
    Average 99 stars, based on 246 article reviews
    monarch hmw dna extraction kit - by Bioz Stars, 2026-10
    99/100 stars

    Images

    1) Product Images from "Resolving missing human polymorphic inversions and other complex variants from ultralong read data"

    Article Title: Resolving missing human polymorphic inversions and other complex variants from ultralong read data

    Journal: Genome Research

    doi: 10.1101/gr.280867.125

    Summary of ONT sequencing experiment results. ( A ) Comparison of the N50 value and the total output of each sequencing run of the four analyzed samples (in different colors). Diverse sequencing protocols using whole-genome sequencing (WGS) or adaptive sampling (AS2/AS3) and DNA isolation methods (Monarch HMW or phenol) are represented by different shapes, whereas empty symbols indicate the use of fresh cells as starting material. ( B ) Enrichment ratio between the read depth inside and outside the targeted regions (depth in/out) of each sample for diverse types of sequencing (black dots), showing a six- to sevenfold average enrichment in AS2 or AS3 compared with WGS (colored bars). ( C ) Average proportion of genotypes obtained for the analyzed inversion regions normalized to the number of AS2 or AS3 targets (percentage, colored bars). Values for each sample (black dots) show considerable variation due mainly to sequencing output differences.
    Figure Legend Snippet: Summary of ONT sequencing experiment results. ( A ) Comparison of the N50 value and the total output of each sequencing run of the four analyzed samples (in different colors). Diverse sequencing protocols using whole-genome sequencing (WGS) or adaptive sampling (AS2/AS3) and DNA isolation methods (Monarch HMW or phenol) are represented by different shapes, whereas empty symbols indicate the use of fresh cells as starting material. ( B ) Enrichment ratio between the read depth inside and outside the targeted regions (depth in/out) of each sample for diverse types of sequencing (black dots), showing a six- to sevenfold average enrichment in AS2 or AS3 compared with WGS (colored bars). ( C ) Average proportion of genotypes obtained for the analyzed inversion regions normalized to the number of AS2 or AS3 targets (percentage, colored bars). Values for each sample (black dots) show considerable variation due mainly to sequencing output differences.

    Techniques Used: Sequencing, Comparison, Whole-Genome Sequencing, Sampling, DNA Extraction

    Related Articles

    Isolation:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6). .. BJ and IMR-90 cells were used as input for gDNA isolation using the NEB Monarch HMW DNA extraction kit for cells and blood (New England Biolabs, Cat. No. T3050L) following manufacturer’s instructions. .. HMW DNA was quantified by Nanodrop and QubitTM 1xdsDNA High Sensitivity kit (Thermo Fisher, Cat. No. Q33231 ).

    DNA Extraction:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6). .. BJ and IMR-90 cells were used as input for gDNA isolation using the NEB Monarch HMW DNA extraction kit for cells and blood (New England Biolabs, Cat. No. T3050L) following manufacturer’s instructions. .. HMW DNA was quantified by Nanodrop and QubitTM 1xdsDNA High Sensitivity kit (Thermo Fisher, Cat. No. Q33231 ).

    Article Title: Cultural transmission and genomic co-divergence in the willow tit across the Palearctic
    Article Snippet: .. High-molecular-weight DNA for PacBio HiFi sequencing was extracted with the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs). .. Illumina short-read libraries were prepared from the same individual.

    Article Title: The nuclear and mitochondrial genomes of the lined rockskipper, Istiblennius lineatus (Teleostei: Blenniidae).
    Article Snippet: .. 13 14 DNA was extracted from muscle tissue using the New England Biolabs High Molecular 15 Weight DNA extraction kit (Cat. #T3050L) following the manufacturer’s instructions. .. 16 Genomic DNA was sequenced on a Pacific Biosciences’ Revio 25M SMRT cell (HiFi 17 protocol) by the Uppsala Genome Centre, NGI-SciLifeLab (Uppsala, Sweden).

    Article Title: Tregs transduced with a Ro60-reactive TCR have increased immunosuppressive functions in Sjögren’s disease
    Article Snippet: ImmunoCult DC Differentiation Kit , StemCell Technologies , Cat No. 10985. .. Genomic DNA Extraction Kit , New England Biolabs , Cat No. T3010L. .. Lenti-X Provirus Quantitation Kit , Takara , Cat No. 631239.

    Article Title: MethylBench: A comprehensive benchmark of DNA methylation profiling methods across diverse sequencing platforms
    Article Snippet: .. DNA was extracted using the Monarch® HMW DNA Extraction Kit for Cells & Blood (New England Biolabs). .. DNA from EDTA blood of 5 probands was extracted using the Flexigene kit (Qiagen) according to manufacturer’s instructions.

    Article Title: Structural Genomic Variation and Its Potential Role in Deer Speciation
    Article Snippet: .. DNA was extracted using the NEB Monarch HMW DNA Extraction Kit for Cells & Blood (#T3050) UHMW protocol, with some modifications to the cell lysis step. .. Specifically, cells were digested at 56°C for 1 h at 300 rpm with 100 μL Nuclei Prep Buffer, 100 μL Nuclei Lysis Buffer, 10 μL Proteinase K (NEB #P8107, 20 mg/mL), and 10 μL 1 M DTT (GoldBio #DTT in dH2O, final concentration ~50 mM).

    Sequencing:

    Article Title: Cultural transmission and genomic co-divergence in the willow tit across the Palearctic
    Article Snippet: .. High-molecular-weight DNA for PacBio HiFi sequencing was extracted with the Monarch HMW DNA Extraction Kit for Cells & Blood (New England Biolabs). .. Illumina short-read libraries were prepared from the same individual.

    Lysis:

    Article Title: Structural Genomic Variation and Its Potential Role in Deer Speciation
    Article Snippet: .. DNA was extracted using the NEB Monarch HMW DNA Extraction Kit for Cells & Blood (#T3050) UHMW protocol, with some modifications to the cell lysis step. .. Specifically, cells were digested at 56°C for 1 h at 300 rpm with 100 μL Nuclei Prep Buffer, 100 μL Nuclei Lysis Buffer, 10 μL Proteinase K (NEB #P8107, 20 mg/mL), and 10 μL 1 M DTT (GoldBio #DTT in dH2O, final concentration ~50 mM).



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    Image Search Results


    Summary of ONT sequencing experiment results. ( A ) Comparison of the N50 value and the total output of each sequencing run of the four analyzed samples (in different colors). Diverse sequencing protocols using whole-genome sequencing (WGS) or adaptive sampling (AS2/AS3) and DNA isolation methods (Monarch HMW or phenol) are represented by different shapes, whereas empty symbols indicate the use of fresh cells as starting material. ( B ) Enrichment ratio between the read depth inside and outside the targeted regions (depth in/out) of each sample for diverse types of sequencing (black dots), showing a six- to sevenfold average enrichment in AS2 or AS3 compared with WGS (colored bars). ( C ) Average proportion of genotypes obtained for the analyzed inversion regions normalized to the number of AS2 or AS3 targets (percentage, colored bars). Values for each sample (black dots) show considerable variation due mainly to sequencing output differences.

    Journal: Genome Research

    Article Title: Resolving missing human polymorphic inversions and other complex variants from ultralong read data

    doi: 10.1101/gr.280867.125

    Figure Lengend Snippet: Summary of ONT sequencing experiment results. ( A ) Comparison of the N50 value and the total output of each sequencing run of the four analyzed samples (in different colors). Diverse sequencing protocols using whole-genome sequencing (WGS) or adaptive sampling (AS2/AS3) and DNA isolation methods (Monarch HMW or phenol) are represented by different shapes, whereas empty symbols indicate the use of fresh cells as starting material. ( B ) Enrichment ratio between the read depth inside and outside the targeted regions (depth in/out) of each sample for diverse types of sequencing (black dots), showing a six- to sevenfold average enrichment in AS2 or AS3 compared with WGS (colored bars). ( C ) Average proportion of genotypes obtained for the analyzed inversion regions normalized to the number of AS2 or AS3 targets (percentage, colored bars). Values for each sample (black dots) show considerable variation due mainly to sequencing output differences.

    Article Snippet: High-molecular-weight genomic DNA was obtained by two different methods following the protocol specifications: (1) the Monarch HMW DNA extraction kit for cells & blood (New England Biolabs) starting from about 6 million cells and (2) a phenol-based isolation protocol optimized to obtain ultralong reads for ONT sequencing ( ) starting from 20 to 30 million cells.

    Techniques: Sequencing, Comparison, Whole-Genome Sequencing, Sampling, DNA Extraction